USA > Ohio > Hancock County > Findlay > Twentieth Century History of Findlay and Hancock County, Ohio, and Representative Citizens > Part 133
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Digitized by
-
wauw underwent any Characteristic changes either following the sensitizing injec- ion or during the local anaphylactic reaction produced by the atradermal injection of 0.01 cc. horse serum.
A considerable number of observations have been pub- ished on changes in the leucocytes following the injection of arious antitoxic sera and during the anaphylactic reaction in uman beings, a very good summary of which appears by Bianco Bienenfeld (Jahrbuch für Kinderheilkunde und Phy- iche Erziehung, N. F., Bd. LXV, 1907). Bienenfeld's per- onal observations showed a leucopenia, immediately following he injection of large doses of serum, followed by a leuco- ytosis, which, in most cases of the serum disease, in turn gave lace to a recurrence of leucopenia. The leucopenia is as- ribed to negative chemotaxis and is characterized by a iminution of the polymorphonuclear neutrophiles. The non- ranular cells seem much more resistant to these changes than o the granular cells, since they undergo much less diminution. 'he eosinophiles are not affected. Bienenfeld states that toward he end of the leucopenia there appear many large mono- uclear and transitional cells. These are ascribed to regenera- ive processes in the blood and lead to the belief that during ne serum disease there is destruction of leucocytes followed y regeneration; however, owing to lack of more positive roof, the author leaves the question open as to the cause of ne leucopenia, whether it is due to non-functionating of the lood-forming organs or to a destruction of leucocytes.
Hamburger and v. Reuss (Ueber die Wirkung artfremden enuinen Eiweisses auf die Leukozyten. Zeitschrift für Biol- gie, 1910, XLVII, 24) in a large series of experiments carried ut on rabbits found that the intravenous injection of foreign roteids is followed in about five minutes by a marked diminu- on in the number of leucocytes. An exception to this rule as found in the case of horse serum which caused practically le same result as the injection of homologous (rabbit) serum, amely, a hyperleucocytosis. The same result, hyperleuco- tosis, followed the injection of normal salt solution and in- ed simple venepuncture without any injection. The authors nsider the hypoleucocytosis following the injection of reign proteid, the result of a toxic action.
Aschenheim (Ueber Schwankungen der Leukocytenzahle ch, Traumen und Injektionen. Zeitschrift für Biologie, 1910, [, 385) injected a series of rabbits with homologous and het- ologous serum and in opposition to Hamburger and v. Reuss served hypoleucocytosis following the homologous, as well as : heterologous serum. Simple venepuncture, without injection any kind, was followed by hyperleucocytosis. Aschenheim s unable to refer the effect on the leucocyte count of simple nepuncture to reflex vasomotor changes due to painful muli; since anesthesia did not prevent the occurrence of perleucocytosis. He admits, however, that his experiments re not conclusive since the sympathetic system may not have en inactive under anesthesia.
Subcutaneous injections into human beings of human serum 1 serum from a variety of animals, produced changes in the cocyte count which followed no definite rule.
until after many counts had been made, we feel that in none of the work which we have seen reported, has enough care been bestowed upon the establishment of the variations in the normal leucocyte counts in rabbits. It was not until after we had obtained many confusing results that we devoted our attention seriously to this point.
Before giving the results of our counts a brief description of the conditions under which the work was carried out will be given.
Young adult rabbits about a year old and of approximately the same size and weight were selected from a large number of country-raised rabbits. The males were separated from the females throughout the experiments and no pregnant females were used.
In the first series tested, as many as four or five rabbits were kept in a three- by four-foot cage, but in the subsequent series each rabbit was kept in a separate cage in order to pre- vent fighting, which might result in injuries or infections and thus influence the leucocytes.
All rabbits were fed once a day at approximately the same hour, 10 a. m. The food consisted of greens, grain and hay in such quantity that all would usually be consumed in four to six hours after feeding.
The blood for counting was taken from an ear vein and to insure a free flow of blood, after the ear was shaved, cleaned with alcohol and dried, a test tube containing warm water was placed beneath the ear; this caused a prompt dilatation of the veins and free flow of blood. As soon as the pipette for counting had been filled, smears for the differential counts were made.
Thoma-Zeiss blood counting apparatus was used; the pi- pettes were of the large, 1-20 dilution variety, the diluent was prepared fresh each day by adding four drops of glacial acetic acid to 40 cc. distilled water. In all cases two slides of five square millimeters each were counted in order to determine the total number of leucocytes.
The films for the differential counts were stained with Hast- ings' stain and a total of 200 cells counted for each differ- ential. Duplicate counts made in a number of instances showed that this number of cells gave percentages which agreed quite closely with those given by counting 400 cells or more.
Since the value of this work depends entirely upon the ac- curacy of the counts we have attempted to get some idea of the percentage error in our counts. To this end we made duplicate determinations at frequent intervals throughout the work. A free flow of blood having been obtained from the ear vein, two pipettes were filled in rapid succession and two slides of five square millimeters each counted from each pipette. The average percentage variation of the two counts from the mean of the two counts in 48 such duplicate determinations was 7.5 per cent.
We further tried to get an idea of the accuracy of the counts by determining the percentage variation of each slide from the average of the two slides. In 174 such determinations the average percentage variation between slides was 3.9 per cent.
Digitized by
260
JOHNS HOPKINS HOSPITAL BULLETIN.
[No. :
4pm
8pm
20 122
21 /27
22 /2
4pm
8 pm
Feb 1 12m
3 /20
16000
15000
- Inec
14000
13000
12.000
11000
9000
8000
6000
Jose
Rabbit 52
4000
/7 .
3000
75
2000
1000
CHART I .- TOTAL LEUCOCYTE COUNTS.
Jan. 19 injected normal horse serum intravenously as follows: Rabbit No. 52, 0.1 cc .; Rabbit No. 17, 1 cc .; Rabbit No. 75, 5 cc. Jan. 31 injected 0.01 cc. normal horse serum intradermally in all three rabbits.
+INDc Jan 19 12'h
4 pm
20 21
2/ 120
22 /2»
4pm
Spm
Feb 1 127
3
- INOC
Total Wie
---
9000
8000
7000
6000
5000
2000
1000
CHART III.
Rabbit No. 17 showing total leucocyte count and absolute rules for polymorphonuclear, large and small mononuclear leucocytes
- INOC Jan 19 in
8pm
2,
22 25
Feb 1 /2
2
/2000
Total Was
11000
PAN
SM
1000
-- -
9000
8000
6000
5000
2000
1000
CHART II. Rabbit No. 52 showing total leucocyte count and absolute values for polymorphonuclear, large and small mononuclear leucocytes.
4 pm
5pm
20 21
122
22 /2^
1290
4pm ipM
12M
N
5
Feb
Total war
Sm ...... LM - -
10000
9000
8000
7000
6000
4000
3000
2000
1000
CHART IV. Rabbit No. 75 showing total leucocyte count and absolute ralces for polymorphonuclear, large and small mononuclear leucocytes
Digitized by
Jan 19 12x
1
3/
21
5
Inec Jandi An
...
he results of the counts made from the separate pipettes are bulated below :
Rabbit No. 23, March 14, 1910
Rabbit A
99, May 19,
Rabbit No. 99. May 20,
t pipette
8670
7360
4100
3540
pipette
8660
7880
4020
4060
pipette
8470
7720
3920
3560
h pipette
4220
4060
h pipette
3780
3700
Average
8600
7653
4008
3784
'eatest variation
from average ....
1.5%
3.8%
5.4%
7.3%
The above percentages represent all the data we have on e accuracy of the counts and we feel they indicate that our sults may be taken as trustworthy.
FIRST SERIES.
bbit No. 52. January 19, 1910, injected 0.1 cc. normal horse serum intravenously. January 81, 1910, cutaneous anaphylactic test, 0.01 cc. normal horse serum intradermally. Local reaction negative.
Total
Pmn*
Lm
8m
Eos
Mst
ore injection ... 10900
22.5 2452
15.0
1685
57.5 6268
8.5
381
1.5
164
hours after ...
7000
47.4 8318
6.6
462
89.4 2758
4.0
290
2.4
168
9100
55.6 5060
6.8
574
32.6 2966
1.6
146
8.6
328
12000
82.8
3876
6.0
5.5
440
63.0
5040
2.5
200
2.0
160 174
ore test
8200
52.8
7900
86.6
2892
8.6
680
51.3
4058
2.6
1.6
0.8
24
2.8
184
..
8500
25.0 2125
14.0 1190
57.0 4845
4.0
104 340
3.0
207
>bit No. 17. January 19, 1910, injected 1.0 cc. normal horse serum intravenously. January 81, 1910, cutaneous anaphylactic test, 0.01 cc. normal horse serum intradermally. Local reaction doubtful.
Total
Pmn
Lm
Sm Total % Total
EOB Total
Mst % Total
ore injection ... 11800 hours after. 6100
54.6 8381
5.0
805
84.0 2074
5.3
324
1.0
61
..
10100
48.3
4879
4.6
465
43.0
4343
3.0
803
1.0
101
=
9000
..
8000
29.6 2868
10.4
882
58.2
4256
8.2
256
3.6
288
Rabbit No. 15
Total
Pmn
Lm
Sm
Eos
Mst
1910
Leucocytes % Total
% Total % Total
% Total % Total
249 March 2, 9 a. m.
10195
86.0 8670
8.5
866
52.5 5852
1.0
102
2.0 204
1 p. m.
9965
36.0
3587
11.5
1145
47.5 4738
0.5
50
5.5
631
8, 9 a. m.
9875
28.0 2765
9.0
888
57.5 5678
1.0
99
4.5 434
4, 9 a. m.
9520
29.0 2760
10.0
952
57.0 5426
51.5 5711 8.5
888
4.5 499
March 16, 9 a. m.
10400 12580
40.22 4191
16.88 1755
86.88 8836
0.5
63
6.0
5 p. m.
12800
51.5
6592
10.0
1280
82.5
4160
0.5
64
5.5
17, 9 a. m.
11280
83.0 8722
9.5
1071
53.5 6084
48.0 4704
1.0 1.0
.. 99 95 .
5.0
1 p. m.
9080
28.5
2582
16.0
1449
53.5 4847
0.6
45 1.5
894 186 858
23, 9 a. m.
9880
81.0
8062
4.5
444
60.5
5977
0.5
49
3.5
345
24, 9 a. m.
11660
81.0 8614
4.5
524
60.0
6996
. .
4.5
524
April
6, 9 a. m.
9920
89.0 8868
12.0
1.190 45.5 4518
..
..
re test.
8200
87.2 8050
8.8
722
49.2
4085
1.6
181
8.2
262
lours after ..
8400
42.4
8562
10.8
907
48.2
8829
0.8
67
2.8
285
(Inoc. 0.01 cc.
14460
46.5 6724
4.0
578
45.5
6579
..
2.5
880
7, 9 a. m.
11100
41.0 4551
13.5
1490
41.0
4551
..
8.0
309
9, 9 a. m.
9700
41.5 4025
14.5 1406
89.5 8831
..
..
4.5
436
Rabbit No. 24
March 2, 9 a. m.
5905 8360
89.0
8260
18.5
1128
44.0
3678 4514
0.5 0.5
40
4.5
359
5 p. m.
7990
29.5
2357
9.0
719
56.5
8175
8837
..
:
4.0 2.5
189
5, 9 a. m.
5500
84.5
1928
7.5
419
1198
56.0 4972 4690
4292
4.5
460
6.0
1.6
114
Sm=small mononuclears.
Eos=eosinophiles.
Mst= mast cells.
19, 9 a. m.
7160
82.0 2291
12.0
859 50.0 8580
3.0 3.0
225 214
3.0
150 214
18, 9 a. m.
7500
83.5 2512
9.5
712
52.0
3900
4.4
469
3.6
389 618
Lm=large mononuclears.
5 p. m.
17, 9 a. m.
7140
22.4
1599
16.0
1142
54.4 8884
5.6
400
2.0
499
8, 9 a. m.
10800
27.0 2781
6.0
618
64.0 6592 ..
..
....
7700
86.0 2772
7.5
578
58.8 4104
1.8
100
2.0 1.9 147
'he variations which the leucocytes have undergone are e readily seen if the above figures are plotted out in the n of curves. Chart I shows the total leucocyte count for
1 all the tables the following abbreviations are used :
Wbc=white blood cells.
Pmn=polymorphonuclears.
1 p. m.
8880 10660
20.5
1820
18.5
12.8
1864
10.5
1078
55.5 3102
4.0
855
6.0
532
22, 9 a. m.
6520
27.5
1793
11.25
788
55.75 8635
54
8200
40.0
8280
13.0
1066
48.0
8526
2.0
164
2.0
164
Lm
Sm
Eos
Mst
Leucocytes % Total
%
Total % Total
%
Total
% Total
re injection ... 9700
82.7 8753
5.7
552
46.7 4529
7.0
679
1.8
174
hours after.
8100
57.3 4641
6.0
486
82.0 2592
4.0
824
0.7
56
9400
46.6 4880
5.6
526
42.8 8976
4.6
432
0.7
65
9700
..
...
8800
40.8
8988
10.0
830
46.8
8884
1.6
198
0.8
180
5 p. m.
15200
22.5 3420
6.5
988
68.5 10412 . .
8100
85.6
2884
10.4
842
52.0
4212
0.4
82
1.6
190
=
"
..
....
6600
25.5
1689
19.5
1287
49.5 3267
8.0
198
2.5 165
....
6700
21.5 1440
22.5 1508
51.0 8417
1.5
100
8.5 285
4.0 880
5, 9 a. m.
11090
82.5 8604
8.0
887
1029
1051
50.0 4780
52.5 5029
..
4.0
704 451 490 525 479
March 21, 9 a. m.
9580 9860
88.0
8746
11.0
1084
47.0
4684
145
9000
42.6
8884
9.0
810
44.6
4014
8.0
270
0.6
160
hours after.
8300
84.3
2847
21.8
1770
89.0
8287
4832
0.8
64
48
0.8 2.0
190
5 p. m.
11490
40.0
4596
7.0
804
47.5 5457
2.6
812
..
8600
27.6 2378
14.3 1230
52.0 4477
4.0
844
2.0
771
85.0 2870
1.5
124
1.6
182
hours after ...
Lost
42.8
8000
22.0 1760
11.0
880
64.3 5144
55.5 8829
1.5
4.0
860
1.0
90
›re test
8000
80.6 2448
14.8
1144
51.6 4128
1.8 2.8
104
191
3.
8000
20.0
1600
18.0
1440
18.0
1080
55.2 8812
0.8
853
3.6
858
..
9800
58.8 5714
8.6
853
7.6
684
51.0
4590
..
..
bit No. 75. January 19, 1910, injected 5.0 cc. normal horse serum intravenously. January 31, 1910, cutaneous anaphylactic test, 0.01 cc. normal horse serum intradermally. Local reaction positive.
Total
Pmn
18, 9 a. m.
9800
85.5 8479
82.5 8107
11.0
1983
51.5
6478
6.0 624 754
1 p. m.
81.0 8899
80.5 2922
12.0 1149
5 p. m.
8.2
310
1.5
5.5
4.0
578
1 p. m.
1 p. m.
5620
27.5
1545
15.0
848
11.0
692
61.0
. .
1.0
58
3, 9 a. m.
251
4, 9 a. m.
6290
24.0
1509
85.2 3752
87.0 8781
10220
Digitized by
- -
VIVu of Hoimar forse serum the leucocyte count had fallen in all the rabbits. The greatest drop is in rabbit No. 17, which received 1 cc. serum; the next greatest drop is in No. 52 which received 0.1 cc. serum; while the leucocytes of No. 75, which received 5 cc. serum, showed the smallest drop. Eight hours after the inoculation the leucocytes of all three rabbits had risen but not to their original levels. At the end of 24 hours the leucocytes of No. 52 slightly exceeded their original level and from this point on to the end of 72 hours all of the curves fell.
Following the intradermal injection of 0.01 cc. serum there was practically no change in the total leucocyte counts at the end of four and eight hours, in any of the rabbits. At the end of 48 hours the leucocytes of No. 75 were slightly in- creased. This rabbit was the only one of the series which gave a well marked positive reaction.
Charts II, III and IV show the total leucocyte count, the total number of polymorphonuclears, small and large mono- nuclears plotted out separately for each rabbit. It is evident from these three charts that the low leucocyte counts, four hours after inoculation, are due to a fall in the small mono- nuclears; this fall being great enough to bring the total count down in spite of a slight increase in the polymorphonuclears in the case of Nos. 52 and 75. In the case of No. 52, eight hours after inoculation the increase in the total count is due largely to an increase in the polymorphonuclears, while the still further increase in the total count at the end of 24 hours is due to a recovery of the small mononuclears which elements carry the curve to its high point in spite of a falling off of the polymorphonuclears at this time. There seems to be nothing noteworthy about the differential counts following the in- tradermal inoculation.
..
8000
27.0 2160
25.0 1725
15.0 1085
720
57.6 6912
1.8
156
206
0.6 1.2
48
Leucocytes % Total
29.8 8517
9.0
1062
46.9 5535
9.4
1110
4.7
555
=
6000
24.0
1440
51.0 4947
6.0
588
88.2 8705
760
49.0 4655
....
9500
41.0 8895
8.0
9.2
45.2
..
%
Total
% Total
Leucocytes % Total
6900
..
Rabbit No.
1910
1910
%
%
8.6
80.6 2999
86.3 8267
SECOND SERIES.
19, 9 a. m.
9560
serum intradermally.)
March 16, 9 a. m.
.
41
3.0 250
4287
9.4
Total % Total
2.0
4.5 448
4.0 5.0 5.5
4.5
8.5 847
11.0
10.5
56.5
44.0 42.0
60.4
262
JOHNS HOPKINS HOSPITAL BULLETIN.
[No. 5g
Two periods of observation before inoculation
Mch. 16-19
Mch. 21-24
Apr. H
Highest count .. 11490
12800
11600
1500
23, 9 a. m.
5320
27.0 1436
5.5
292
59.5
3164
3.0
159
5.0
266
24, 9 a. m.
5820
30.5 1775
9.5
553
51.5
2997
3.0
174
5.5
329
April 6, 9 a. m. 7100
Variation
1970
3240
5140
550%
Rabbit No. 24:
Highest count ..
8360
10660
8680
894.
Lowest count ..
5620
7140
5320
-
7580
57.0
4820
8.6
644
33.5 38.0
1.0
96
0.5
48
5 p. m.
9670
63.5
6140
6.0
580
26.5
2562
4.0
387
4, 9 a. m.
11160
51.5 5747
76.0
8595
2.5
282
19.5
2205
0.5
50
1.5
169
Lowest count ..
6840
6240
6220
5340
5 p. m.
6880
46.5
3199
2680
13.5
1026
50.5
3838
1.0
76
18, 9 a. m.
6460
56.5 3650
4.5
290
36.5
2358
32
2.0
129
March 21, 9 a. m.
6740
87.0 2494
9.5
640
52.0 3505
0.5
33
1.0
67
1 p. m.
6220
41.5 2581
6.5
404
6.0
446
48.0 85.5
2985 2641
1.5
93 2.5
155
22, 9 a. m.
6780
42.0 2847
39.0 8221
30.0 1878
4.0
250
61.0 3819 0.6
31
4.5
282
April 6, 9 a. m.
6920
38.5 2818
4.5
311
62.0
4290
..
..
..
1 p. m.
10060
53.0
5339
5.5
559
40.0
4024 4764
0.5
0.5
2.5
158
9, 9 a. m.
5320
84.0 1809
5.0
266
58.5
3112
0.5 0.5
40 82 42 26
1.0 2.0
106
Rabbit No. 104
March 2, 9 a. m.
7880
87.0
2915
8.5
670
51.5
4058
236
5 p. m.
10340
86.0
3722
11.5
1189
46.0
4756
6.5
672
4, 9 a. m.
7160
33.5
2398
6.0
429
55.5
8974
0.5
36
4.5
822 270
March 16, 9 a. m.
8860
25.5 2259
8.0 7.5
709
57.5
5094
1.0 5.5
83
8.0
709
5 p. m.
18480
35.5
4785
12.5
1685
45.5
6133
2.F
837
4.0
539
18, 9 a. m.
12180
87.0
4506
35.5
3685
12.0
1245
89.0
4048
8.6
882
5.0 519
March 21, 9 a. m.
5900
22.0 1298
14.5
855
55.5
3274
3.0
177
5.0 295
1 p. m.
9480
66.0 6256
4.5
426
26.5
2512
1.5 7.5
142
1.5
142 77
23, 9 a. m.
7940
45.0
8578
10.5
883
38.5
3057
1.0 0.5
79 42
5.0
394
April 6, 9 a. m.
6280
38.5 2417
10.0
628
274
24.5
2685
0.5
54
2.5
274
8, 9 a. m.
8820
82.0 2822
8.0
705
56.0
4989
0.5
46
1.0
92
9, 9 a. m.
8040
35.5 2854
4.0
321
56.5
4542
1.0
80
3.0
241
6300
12.0 756 16.0 1008
65.0 4095
8.5
220
3.5
220
1 p. m.
7500
35.5 2662
13.0
975
43.5
3262
1.0
75
7.0 3.5
303
22, 9 a. m.
5900
27.5 1622
12.0
708
56.0
3304
0.5
29
4.0
236
Lowest count ..
9520
9560
6520
3.5 248
1 p. m.
7660
44.0 3370
8.0
612
42.5
3255
1.5
1.0
2.0
135
0.5
34
9, 9 a. m.
5680 7000
34.0 1931
7.5
426
52.0
2953
4.0 2.0
140
4.0 280
Rabbit No. 92
Variation
2740
3520
3360
76
1 p. m.
9635
51.5
4962
9.0
867
6.5
444
6.5
725
40.0
4464
1.0
111
1.0
111
March 16, 9 a. m.
6240 8040
48.0
2995
9.0
10.0
80
52.5
4221
0.5
40
2.5
201
Variation
4470
3300
2040
4745
17, 9 a. m.
9540
27.0 2576
9.5
906
60.5
5772
48
2.5
238
Rabbit No. 104:
Highest count .. 10650
12180
9480
10960
Lowest count ..
7160
8760
5900
5 p. m.
7440
56.0
4166
11.0
746
44.5
3017
..
..
2.5
169
Variation
3490
3420
3580
468
100
7, 9 a. m.
6300
52.0
3276
10.5
661
34.5
2173
8, 9 a. m.
8360
45.0
3762
2745
6.5
585
58.5
5265
1.5
135
8.0
3.0
83
17, 9 a. m.
8760
88.0 2891
8.0
701
50.0
4380
4.5
365
8.0
974
5 p. m.
7700
46.5
8580
7.5
577
37.5
2887
4194
8.0
270
2.5
225
24, 9 a. m.
8520
41.5
8535
11.0
937
89.0
3322
1.0
62
6.0
376
5 p. m.
10640
60.5 6437
2.0
212
84.5
3670
0.25
26
2.75
292
353
Chart V represents the total leucocyte counts for the four rabbits of the third series plotted out in such a way that one can compare the variations in the whole series at a glance. This chart shows that the variations in the leucocytes during the two periods of observation before inoculation, March 2-5 and March 16-19 were approximately as great as those following the intravenous injection of serum, March 21, and the cutaneous test, April 6, except in the case of rabbit No. 15, which was a control and did not receive the intravenous inoculation with the others on March 16. This is made clear by the following figures :
Rabbit No. 15:
525
5 p. m.
8880
47.0 4079
8.0
694
38.5
3341
3.0
260
142
115 89
4.0
309
5 p. m.
8940
30.5 2726
3.0
268
64.5
5766
1.0
89
7, 9 a. m.
6760
41.0 2771
5.5
372
51.0
3447
227
2.5
142
7.5
525
41.0
2870
Eos
Mst
Total Pmn Leucocytes % Total
Lm Sm % Total % Total
% Total
% Total
March 21, 9 a. m.
(Inoc. 0.01 cc. serum intravenously.)
27.0 1917
5.5
390
62.0
4402
2.0
2589
1.0
Rabbit No. 92:
Highest count .. 11310
9540
8260
10021
561
37.0
2308
1.5
93
4.5
280
1 p. m.
$4.5
2778
9.0
619
42.0
2889
..
..
2.5
172
9 a. m.
7600
35.0
36.5 2898
4318
6.0
639
49.0
5218
1.0
106
3.0 3.5
372
3, 9 a. m.
7460
56.0
4177
4.0
298
37.0 2760
8.0
223
1 p. m.
11160
42.0
4687
837
42.0 4687
613
4.5
894
19, 9 a. m.
10880
intravenously.)
22, 9 a. m.
9020
86.5
8292
11.5 1037
46.5
44.5 2794
1 p. m.
10980
70.0 7672
2.5
7, 9 a. m.
9200
58.0 5336
8.0
738
32.5
2900
..
..
This chart (V) shows in all cases an increase in the lets- cytes at the count made either three hours or seven box. after feeding, with a fall by the next morning at 9 o'clock somewhere near the original level. It seems quite reasonzal to regard this increase as a digestive leucocytosis. The effet" if any, of the intravenous injection of serum, March 21, L'. the intradermal inoculation, April 6, is to depress to a slice: extent the height of this digestive leucocytosis.
Charts VI, VII, VIII and IX represent the total number leucocytes and absolute values of the polymorphonucles: large, and small mononuclear leucocytes for rabbits Nos. li. 24, 92 and 104, respectively. These charts show that in ger- eral an increase in the total leucocyte count is due to an it- crease in the two principal elements, the polymorphonucles" and small mononuclear leucocytes. In this series howere :. the polymorphonuclears as a rule undergo a greater increas than do the small mononuclears.
In order to determine if there is anything characteris: in the differential count following the intravenous injectie: of fairly large amounts of serum or the intradermal injecties. of minimal amounts we have analyzed the changes in the tote counts and the number of polymorphonuclears and sma? mononuclears three hours and seven hours after injectier For purposes of comparison we have included the correspon- ing counts made before injection. The results are shown in the accompanying table, examination of which shows that a: increase in the total count after the injection of serum my: be due to an increase of either or both of the principal lec.". cytes and that this relationship does not differ from that es- tering into increases in the total count which occurred befe .. the injection of any serum.
* Rabbit No. 15 was a control and did not receive an intrarenoci injection.
Digitized by
2.5
206
(Inoc. 0.01 cc. serum intradermally.)
0.5
50
1.0 0.5
40
8.0
689
45.5
3804
2.5
186
23, 9 a. m.
8260
8.0
661
50.5
4171
24, 9 a. m.
6840
61.0
4172
31.5
2154
1.0
68
5, 9 a. m.
11810
6260
7940
5 p. m.
1 p. m.
10650
40.5
5, 9 a. m.
9000
80.5
10.5 1279
41.5
5054
8.0
577
1.0
8.0
684
1
2.5
108
60.0
3861
8, 9 a. m.
(Inoc. 0.01 cc. serum intradermally.)
Mch. 2-5
Following Following intravenous intraderzi inoculation* inoculóz
-
8, 9 a. m.
45.5 3185
March 2, 9 a. m.
0.5 0.5
(Inoc. 1.0 cc. serum intravenously.)
83
(Inoc. 5.0 cc. serum
(Inoc. 0.01 cc. serum intradermally.)
4.0
Rabbit No. 24-Cont.
- Fed Mich 2 9am
1pm
Mens 9am
10% -
24 -
- Fed
-
- Fal Mch 5 9am
Mch 16 9am
-
5 pm
Mch 17 9am
CHART V.
1 pm
5pm
Mch 22 9am
23
9am
24 9am
Ape 6 9am
4-INK
1pm
5pm
5pm
+ Fed Apr7 9am
Ape 8 9am
1
Ape ? 9am.
1000
2000
3000
6000
7000
8000
9000
Mach 2 9am
cytes for entire period of observation.
values of polymorphonuclear, large and small mononuclear leuco-
Rabbit No. 24 showing total leucocyte counts and absolute
Mck 16 9am
Mch 16 9am
1pm
Som
17 9am
18 9am
19 9am
Mich 21 9am
1pm
5pm
22 9am
23 9am
24 9am
Apr 6 9am
+ INDC
1pm
5pm
7 9am
8 9am
9 9am
values of polymorphonuclear, large and small mononuclear leuco-
1000
2000
3000
4000
5000
Como
2000
8000
9000
10000
11000
Mch 2 9am
1pm
Total WBC
5pm
LM-
SM -
PMN .
3 9am
¥ 94m
-
5 9am
5 9am
'pre
Sam
17 9am
1, 9am
19 9am
McÀ 1/ 9am
+INOC
Ipn
Som
22 92m
23 9am
2. 9am
Apr 6 9am
1pm
Sam
7 927
: 9am
9 98m
nal horse serum intradermally.
re period of observation. April 6, 10 a. m., injected 0.01 cc. lymorphonuclear, large and small mononuclear leucocytes for
Lbbit No. 15 showing total leucocyte counts and absolute values
LM
SM
PMN
3 94%
MA 16 9am
Som
17 9am
CHART VI.
18 9am
19 947
Mich 19
9am
Mich 21 9 am
, 24, 92.1. Mich 21 9am
1pm
5pm
22 9am
Mich 23
Mch 24 9pm
Apr 6 9am
+Imes
7 9am
9am
9 Jam
'mal horse serum intradermally in all rabbits.
Rabbits Nos. 15, 24, 92 and 104 showing total leucocyte counts entire period of observation. March 21 injected normal horse um intravenously as follows: Rabbit No. 24, 0.1 cc .; Rabbit . 92, 1 cc .; Rabbit No. 104, 5 cc. April 6 injected 0.01 cc.
cytes for entire period of observation.
Rabbit No. 92 showing total leucocyte counts and absolute
1pm
5pm
LM
PMN-
3 9am
-
4 9am
SM -.....
Total WBC
Digitized by
Total WBC
Spo
Rabbit 15
92
Mch 4 9am
Mek 19
9am
CHART VII.
CHART VIII.
Digitized by
small mononuclear leucocytes for entire period of observation. large mononuclear, eosinophiles and mast cells combined, and Rabbit No. 15 showing percentage values of polymorphonuclear,
10.
20
30
40
50
60
70
80
90
Mal & Jam
MST
SMN
Mch 3 9am
Mch 4 9am
Mch 6 9am
Met 16 9 cm
1 pm
5pm
Mch 17 9am
Mich 18 9am
Mich 21 9am
5 pm
Mch 22 9am
Mch 23 9am
Meh 24 9am
Aps 692
1pm
5pm
Ap- 7 9am
cytes for entire period of observation.
1000
2.000
3000
4000.
5000
6000
7000
8000
9000
10008
1/000
/2000
13000
14000
1000
1000
4000
5000
6000
7000
8000
9000
Mch 2 9 am
Rabbit 15-
4 9am
-----
24 . ..
$ 9am
Mich 16 9am
1pm
5pm
5pm
17 9am
17 9am
18 9am
18
9am
CHART X.
RINOC 87
1pm
5pm
5pm
22 9am
22 9am
23 9am
23 9am
24 9am
6 9am
+ INDC ALL
1pm
5pm
7 9am
8 9am
9 9am
1000
2000
3000
4000
5000
6000
7000
8000
9000
10000
Mch 2 9am
1pm
5pm
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